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The journal AGRICULTURA (A) publishes scientific works from the following fields: animal science, plant production, farm mechanisation, land management, agricultural economics, ecology, biotechnology, microbiology
ISSN 1581-5439

Ashraf Abd -El Aziz ABDEL-MEGEIDa, Aly Rashad ABDEL-MOEMIN, Manal Kamal ABDEL-RAHMANa, Rasha Mahmoud ARAFA
pp. 63-70

The objective of this study was to determine whether the adverse effects of rich fructose diet can be modulated in the presence of common herbs specifically the effects on lipid profile, liver and kidney functions in male albino rats. The rats were divided into two main groups, the first main group (6 rats) fed on basal diet
containing starch, while the second main group (66 rats) was divided into eleven subgroups (6 rats each) and fed on fructose-rich diet for 15 days and then supplemented with different herbs for 30 days. Feed intake was recorded during the experiment, rats were then sacrified and organ weight/body weight percentage were calculated recorded, liver and kidney functions and lipid profile were estimated. A significant reduction (p<0.05) of cholesterol has been noticed among rat groups that fed on fenugreek 2%, nutmeg 2%, and combined herbs 2% compared to positive control. Rat groups that were fed on nutmeg 2%, cardamom 2% and combined herbs 2%, showed a significant reduction of triacylglycerol (p<0.05). Liver and kidney functions were also improved.
Microscopically examined liver and kidney of rats from ginger 2%, nutmeg 1%, cardamom 2% and combined herbs showed normal hepatocytes and fenugreek seeds 2%, cardamom 1%, nutmeg 1%, ginger 2% and combined herbs were revealed to normal renal cells.

Key words: rats, fructose, herbs, kidney and liver functions


Slovenian:

Ali obrok dopolnjen z zelišči lajša simptome bogate prehrane s fruktozo pri podganah?

Cilj raziskave je bil ugotoviti, ali se škodljivi učinki bogate prehrane s fruktozo lahko spremenijo v prisotnosti zelišč še posebno če učinkujejo na profil lipidov, funkcije jeter in ledvic pri moških albino podganah. Podgane so bile razdeljene v dve skupini, prva skupina (6) podgan je bila krmljena z osnovnim obrokom, ki vsebuje škrob, druga skupina (66 podgane) je bila razdeljena na enajst podskupin (vsaka 6 podganah) in krmljenje 15 dni s fruktozo bogatim obrokom in nato z obrokom dopolnjenem z različnimi zelišč še 30 dni. Zauživanje krme se je med poskusom spremljalo, podgane so bile žrtvovane in masa organov/telesne masa so bile zabeležene in ocenjene so bile funkcije jeter, ledvic in profil lipidov. Značilno zmanjšanje (p <0,05) holesterola smo opazili pri podganah, ki so se hranile 2% triplata, muškatni orešček 2%, in v kombinaciji zelišč 2% v primerjavi s pozitivno kontrolo. Podgane, ki so imele v obroku muškatni orešček 2%, kardamom 2% in kombinirani zelišča 2% so imele značilno zmanjšanje triacilglicerola (p <0,05). Delovanje jetra in ledvice se je izboljšalo. Mikroskopsko proučevana jetra in ledvica podgan krmljenih z dodatkom ingverja 2%, muškatnega oreščka 1%, kardamoma 2% in kombiniranih zelišč so imela normalne hepatocite in triplat semena 2%, kardamom 1%, muškatni orešček, 1%, ingver 2% in kombinirani zelišča so pokazale normalne ledvične celic.

Ključne besede: podgane; fruktoza; zelišča; ledvične in jetrne funkcije


REFERENCES

1. Al-Zuhair H, El-Sayeh B, Ameen HA, Al-Shoora H. Pharmacological studies of cardamom oil in animals. Pharmacol. Res. 1996;34:79–82.

2. Anitha Nandhini AT, Balakrishnan SD, Anuradha CV. Taurine improves lipid profile in rats fed a high fructose diet. Nutr. Res. 2002;22:343–54.

3. Arfeen Z, Owen H, Plummer JL, Ilsley AH, Sorby-Adams RA, Doecke CJ. A double-blind randomized controlled trial of ginger for the prevention of postoperative nausea and vomiting. Anaesth. Intensive Care. 1995;23:449–52.

4. Arner P. Free fatty acids–do they play a central role in type 2 diabetes? Diabetes Obes. Metab. 2001;3:S11–S19.

5. Baker N, Chaikoff IL, Schresdek A. Effect of fructose on lipogenesis from lactate and acetate in diabetic liver. J. Biol. Chem. 1952; 194:435–39.

6. Bancroft D, Gamble M. Theory practice of histological Techniques, 6th edition, Churchill Livingstone, Edinburgh, London, Melbourne, 2008. p 53 and 121.

7. Bar-On H, Stein Y. Effect of glucose and fructose administration on lipid metabolism in the rat. J. Nutr. 1968;94:95–101.

8. Basch E, Ulbricht C, Kuo G, Szapary P, Smith M. Therapeutic applications of fenugreek. Altern Med Rev. 2003;8:20–7.

9. Bellomo G, Comstock JP, Wen D. Prolonged fructose feeding and aldose reductase inhibition: effect on the polyol pathway in kidney of normal rats. Proc. Soc. Exp. Biol. Med. 1987;186:348–52.

10. Bergmeyer HU, Brent E. Aminotransferases. In: Methods of enzymatic analysis, Bergmayer HU (ed), Verlag Chemie Weinhein, Academic Press, New York, 1974. p 735,760.

11. Bezerra RMN, Ueno M, Silva M S, Tavares DQ, Carvalho CRO, Saad MJA. A high fructose diet affects the early steps of insulin action in muscle and liver of rats. J. Nutr. 2000;130:1531–5.

12. Bieger WP, Michel G, Barwich D, Biehl K, Wirth A. Diminished insulin receptors on monocytes and erythrocytes in hypertriglyceridemia. Metabolism 1984;33:982–7.

13. Bin-Hafeez B, Haque R, Parvez S, Pandey S, Sayeed I, Raisuddin S. Immunomodulatory effects of fenugreek (Trigonella foenum graecum L.) extract in mice. Int. Immunopharmacol. 2003;3:257–65.

14. Bjorntorp P. Fatty acids, hyperinsulinemia and insulin resistance: which comes first? Current Opinion in Lipidology 1994;5:166–74.

15. Chevallier A. Encyclopedia of herbal medicine. New York (NY): Dorling Kindersley Publishing, Inc., 2000. p. 271.

16. Daly ME, Vale C, Walker M, Alberti KGMM, Mathers JC. Dietary carbohydrates and insulin sensitivity: a review of the evidence and clinical implications. Am. J. Clin. Nutr., 1997;66:1072–85.

17. Dirlewanger M, Schneiter P, Jequier E, Tappy L. Effects of fructose on hepatic glucose metabolism in humans. Am. J. Physiol. 2000;279:E907-E911.

18. Fields M, Ferretti RJ, Judge JM, Reiser S, Smith JC. Effect of different dietary carbohydrate on hepatic enzymes in copper-deficient rats. Proc. Soc. Exp. Biol. Med. 1985;178:362–6.

19. Friedwald WT, Levey RI, Fredrickson DS. Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge. Clin. Chem. 1972;18:499–502.

20. Glatzel H. Physiological aspect of flavour compound. Indian Spices 1968;5:13–21.

21. Glinsmann WH, Bowman BA. The public health significance of dietary fructose. Am. J. Clin. Nutr. 1993;58:820S–823S.

22. Grover JK, Khander S, Vats V, Dhunnoo Y, Das D. Pharmacological studies on Myristica fragrans- Anti-diarrheal, hypnotic, analgesic and hemodynamic (blood pressure) parameters. Methods Find. Exp. Clin. Pharmacol. 2002;24(10):675–80.

23. Hallfrisch J. Metabolic effects of dietary fructose. FASEB J. 1990;4:2652–60.

24. Harborn SB, Baxter H. Phytochemical dictionary. A handbook of bioactive compounds from plants. London, Taylor and Francis, 1995. Fig. 2289.

25. Henry RJ, Sobel C, Kim J. A modified carbonate phosphotungstate method for the determination of uric acid and comparison with the spectrophotometric uricase method. Am. J. Clin. Patho. 1957;128:152–60.

26. Huang YJ, Fang VS, Juan CC, Chou YC, Kwok CF, Ho LT. Amelioration of insulin resistance and hypertension in a fructose-fed rat model with fish oil supplementation. Metabolism 1997;46:1252–8.

27. Houghton PJ, Zarka R, de las Heras B, Hoult JR. Fixed oil of Nigella Sativa and derived thymoquinone inhibit eicosanoid generation in leucocytes and membrane lipid peroxidation. Plant Med. 1995;61:33–6.

28. Janssen J, Laekeman GM, Peiters LA, Totte J, Herman AG, Vlietinck AJ. Nutmeg oil: identification and quantification of its most active constituents as inhibitors of platelet aggregation. J. Ethnopharmacol. 1990;29:179–88.

29. Kiuchi F, Iwakami S, Shibuya M, Hanaoka F, Sankawa U. Inhibition of prostaglandin and leukotriene biosynthesis by gingerols and diarylheptanoids. Chem. Pharm. Bull. 1992;40:387–91.

30. Madar Z, Abel R, Samish S, Arad J. Glucose-lowering effect of fenugreek in non-insulin dependent diabetics. Eur. J. Clin. Nutr. 1988;42:51–4.

31. Morita T, Jinno K, Kawagishi H, Arimoto Y, Suganuma H, Inakuma T. Heptoprotective effect of myristicin from nutmeg (myristica fragrans) on lipopolysaccharide/dgalactosamine-induced liver injury. J. Agric. Food Chem. 2003;51:1560–5.

32. Murakami T, Kishi A, Matsuda H, Yoshikawa M. Medicinal foodstuffs. XVII. Fenugreek seed: structures of new furostanol-type steroid saponins, trigoneosides Xa, Xb, XIb, XIIa, XIIb, and XIIIa, from the seeds of Egyptian Trigonella foenum graecum L. Chem Pharm Bull (Tokyo) 2000;48:994–1000.

33. Okazaki M, Zhang H, Yoshida Y, Ichino K, Nakayama S, Oguchi K. Correlation between plasma fibrinogen and serum lipids in rats with hyperlipidemia induced by cholesterol free-high fructose or high cholesterol diet. J. Nutr. Sci. Vitaminol. (Tokyo) 1994;40:479–89.

34. Okezie IA. Free radicals, antioxidants and international nutrition. Asia Pacific J. Clin. Nutr. 1999;8:53–63.

35. Oya T, Osawa T, Kawakishi S. Spice constituents scavenging free radicals and inhibitory pentosidine formation. In: Amodel system. Biosci. Biotech. Biochem. 1997;61:263–6.

36. Raju J, Gupta D, Rao AR, Yadava PK, Baquer NZ. Trigonella foenum graecum (fenugreek) seed powder improves glucose homeostasis in alloxan diabetic rat tissues by reversing the altered glycolytic, gluconeogenic and lipogenic enzymes. Mol. Cell Biochem. 2001;224:45–51.

37. Ramu S, Shanmuganathan R, Mambakkam K, Ravichandran M, Carani V. Preventive action of food seasoning spices mixture on fructose-induced lipid abnormalities. Asia Pac. J. Clin. Nutr. 2005;14 (4):420–7.

38. Reeves P G, Nielsen F H, Fahey G C. AIN-93 purified diets for laboratory rodents: report of the American Institute of Nutrition ad hoc writing committee on the reformulation of the AIN-76 rodent diet. J. Nutr. 1993;123:1939–51.

39. Reiser S. Effects of dietary sugars in metabolic risk factors associated with heart disease. Nutr. Health 1985;3:203–16.

40. Sharma RD. Hypocholesterolemic effect of fenugreek (T. foenum gmecum). An experimental study in rats. Nutr. Rep. In. 1984;30:221–31.

41. Shen SC, Ko CH, Tseng SW, Tsai SH, ChenYC. Structurally mrelated antitumor effects of flavanones in vitro and in vivo: involvement of caspase 3 activation, p21 gene expression, and reactive oxygen species production. Toxicol. Appl. Pharmacol. 2004;197:84–95.

42. Srivastava KC, Mustafa T. Spices: Antiplatelet activity and prostanoid metabolism. Prostaglandin. Leukot and Essential Fatty Acids 1989;38:255–66.

43. Steel RGD, Torrie JH. Principles and procedures of statistics: A biometrical approach., 2nd edition, New York: Mc-Graw-Hill, 1980. p 633.

44. Sukumaran K, Kuttan R. Inhibition of tobacco-induced mutagenesis by Eugenol and plant extracts. Mutat. Res. 1995;343:25–30.

45. Thorburn AW, Storlien LH, Jenkins AB, Khouri S, Kraegen EW. Fructose-induced in vivo insulin resistance and elevated plasma triglyceride levels in rats. Am. J. Clin. Nutr. 1989;49:1155–63.

46. Todd RG, Martindale W. Extra pharmacopoeia, Twenty Fifth Edition, 1967, p 859.

47. Valette G, Sauvaire Y, Baccou JC, Ribes G. Hypocholesterolaemic effect of fenugreek seeds in dogs. Atherosclerosis 1984;50:105–11.

48. Vutyavanich T, Kraisarin T, Ruangsri R. Ginger for nausea and vomiting in pregnancy: randomized, double-masked, placebo-controlled trial. Obstet. Gynecol. 2001;97:577–82.

49. Wilson J, Kim S, Allen KGD, Baillie R, Clarke SD. Hepatic fatty acid synthase gene transcription is induced by a dietary copper deficiency. Am. J. Physiol. 1997;35:E1124–E1129.

50. Zavaroni I, Sander S, Scott S, Reaven GM. Effect of fructose feeding on insulin secretion and insulin action in the rat. Metabolism 1980;29:970–3.

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