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Peter-Damian Chukwunomso JIWUBA, Ifeanyi Princewill OGBUEWU, Elisha DAUDA, Caritas Chibuike AZUBUIKE

pp. 17-24

ABSTRACT

The responses of 96 day old broilers fed Chromolaena odorata leaf meal (COLM) on blood profile and gut microbial load were studied for 49 days. The birds were randomly assigned to four treatment groups and replicated three times in a completely randomized design. The birds were fed four experimental diets formulated at 0%, 2%, 4% and 6% for the starter phase and 0%, 4%, 8% and 12% of COLM at the finisher phase for T1, T2, T3 and T4 respectively. All the haematological parameters were similar (P>0.05) across the treatment groups except MCH and WBC which were significantly (P< 0.05) higher and better in the treatment groups than the control group. The results on serum chemistry showed significant (P<0.05) difference for total protein, albumin, creatinine and cholesterol across the treatment groups. Cholesterol was significantly (P<0.05) reduced with increasing levels of COLM. Results showed that coliforms and E.coli were consistently higher (P<0.05) among the T1 birds than those fed T2, T3 and T4. It was concluded that, COLM enhanced adequate haematocrit and immune status, hypoglycaemic ability, suppresses the growth of gut pathogenic microorganisms and enhanced the growth of beneficial microorganism in broilers.

Key words: broilers, COLM, leaf meal, gut health, blood profile, medicinal plant

Slovenian:

Krvna slika in mikroflora črevesja brojlerskih piščancev krmljenih z listno moko (Chromolaena odorata)

Proučevani so bili odzivi 96-dnevnih piščancev, ki so bili 49 dni krmljeni z vključevanjem listne moke Chromolaena odorata (COLM) v obrok na profilu njihove krvi in mikroflore črevesja. Piščanci so bili naključno razdeljeni v štiri naključno oblikovane poskusne skupine in tri ponovitve. Piščanci so bili krmljeni s štirimi eksperimentalnimi dietami, s 0%, 2%, 4% in 6% COLM za prvo fazo pitanja - štarter krmo in s 0%, 4%, 8% in 12% COLM v drugi fazi – finišer krmo. Skupine so bile označene kot za T1, T2, T3 in T4. Vsi hematološki parametri so bili statistično neznačilni (P > 0,05) med skupinami, razen pri MCH in WBC, ki so bili značilno (P < 0,05) višji v testiranih skupinah v primerjavi s kontrolo. Rezultati serumske analize so pokazali značilne razlike (P < 0,05) za testirane skupine za lastnosti kot so skupne beljakovine, albumin, kreatin in holesterol v primerjavi s kontrolno skupino. Holesterol je bil značilno (P < 0,05) manjši z naraščajočimi odstotki COLM v krmi piščancev. Rezultati analiz so pokazali, da so
vsebnosti coliformnih mikroorganizmov in E. coli značilno višje (P < 0,05) pri piščancih iz kontrole T1 v primerjavi s T2, T3 in T4 skupino. Ugotovljeno je bilo, da je COLM spodbudno deluje na hematokrit in imunski status, na hipoglikemično sposobnost, zavira rast črevesnih patogenih mikroorganizmov in povečala rast koristnih mikroorganizmov v črevesju pitovnih piščancev.

Ključne besede: pitovni piščanci, COLM, listna moka, zdravje črevesja, krvna slika, zdravilna rastlina

REFERENCES:

1. Adedapo AA, Ogunmiluyi IO, Adeoye AT, Ofuegbe SO, Emikpe BO. Evaluation of the medicinal potential of the methanol leaf extract of Chromolaena odorata in some laboratory animals. J. of Med. Plants Stud. 2016;4:29-37.
2. Akinmoladun AC, Ibukun EO, Dan-Ologe IA. Phytochemical constituents and antioxidant properties of extracts from the leaves of Chromolaena odorata. Sci. Res. Essay 2007;2:191-194.
3. Akinmutumi AH, Akufo A. The effect of graded levels of dietary inclusion of Siam weed Chlomolaena odorata leaf meal in grower rabbits diet in a Tropical environment. J. of Anim. Vet. Advan. 2006;5:700-711.
4. Anon. Guide to the care and use of experimental animals. Vol.1. Canadian Council on Animal Care. Ottawa, Ontario, Canada 1980;85-90.
5. AOAC. Association of Official Analytical Chemists: Official Methods of Analysis. 6th Edition. Washington DC, USA. 2000.
6. Apori SO, Long RJ, Castro FB, Orskov ER. Chemical composition and nutritive value of leaves and stems of tropical weed Chromolaena odorata. Grass Forage Sci. 2000;55:77-81.
7. Bonsu FRK, Kagya-Agyemang JK, Kwenin WKJ, Hope KN, Sekyere FO. Growth Performance, haematological indices and carcass characteristics of broilers fed diet containing different levels of Chromolaena odorata leaf meal. Egerton J. Sci. Technol. 2013;13:115-125.
8. Bryant MP, Burkey LA. Cultural methods and some characteristics of some of the more numerous groups of bacteria in the bovine rumen. J. of Dairy Sci. 1953;36:205-217.
9. Ekenyem BU, Obih TKO, Ohanaka AO, Nwajiuba CU. Effect of partial replacement of Chromolaena odorata for soybean on haematological and serum chemistry of laying birds. Int. J. of Tropical Agric. Food Syst. 2009;33:228-232.
10. Fasuyi AO, Fajemilehin KSO, Aro SO. Nutritional potentials of Siam weed Chromolaena odorata) leaf meal SWLM) on laying hens: biochemical and haematological implications. Pakist. J. Nutrit. 2005;45:336-341.
11. Igboh MN, Ikewuchi CJ, Ikewuchi CC. Chemical Profile of Chromolaena odorata L. King and Robinson) Leaves. Pakist. J. Nutrit. 2009;8:521-524.
12. Ikewuchi JC, Ikewuchi CC. Anti-cholesterolemic Effect of Aqueous Extract of the Leaves of Chromolaena odorata L) King and Robinson Asteraceae. Potential for the Reduction of Cardiovascular Risk. The Pacific J. Sci. Technol. 2011;122:385-391.
13. Jensen BB, Hojberg O, Mikkelsen LL, Hedermann MS, Cannibe N. Enhancing intestinal function to treat and prevent intestinal disease. Proceedings of 9thInternational Symposium Dig. Physiol. In Pigs, Banff, AB. Canada 2003,1:103-119.
14. Jin LZ, Ho YW, Abdulla N, Jalaludin S. Growth performance, intestinal microbial populations and serum cholesterol of broilers fed diets containing Lactobacillus culture. Poultry Sci. 1998;77:1259-1265.
15. Jiwuba PC, Dauda E, Ezenwaka LC, Eluagu CJ. Replacement Value of Maize with Sweet Potato (Ipomoea batata) Root Meal on Growth Performance and Haematogical Characteristics of Broiler Starter Birds. Arch. Curr. Res. Int. 2016a;53:1-7.
16. Jiwuba PC, Ikwunze K, Ume SI, Nsidinanya NO. Performance, apparent nutrient digestibility and cost benefit of West African dwarf goats fed dietary levels of Moringa oleifera leaf meal. J. Advan. Biol. Biotech. 2016b;8:1-9.
17. Jiwuba PC, Ikwunze K, Dauda E, Ugwu DO. Haematological and serum biochemical indices of growing rabbits fed diets containing varying levels of Moringa oleifera leaf meal. Brit. Biotech. J. 2016c;15:1-7.
18. Jiwuba PC, Kuka TT, Kadurumba OE, Nwachukwu CO. Evaluation of haematological and serum biochemical characteristics of Annona muricata (Sour sop) leaf extract
on broiler birds. International J. Res. Stud. Microb. and
Biotech. 2017;3:6-11.
19. Moses SO, Akintayo O, Kamil OY, Labunmi L, Heather EV, Jessika AT, William N. Chemical composition and bioactivity of the essential oil of Chromolaena odorata from Nigeria. Records Nat. Prod. 2010;41:134-137.
20. NRC. Nutrient requirements for poultry. 9th Rev. Edn., National Academy Press, Washington, DC 1994.
21. Nwokolo E. Leaf meals of cassava Manihot esculenta) and Siam weed Eupatorium odoratum) as nutrient sources in poultry diets. Nutri. Report Int. 1987;36:819-826.
22. Ragab HI, Abdel Ati KA, Kijora C, Ibrahim S. Effect of Different Levels of the Processed Lablab purpureus Seeds on Laying Performance, Egg Quality and Serum Parameters.
Int. J. Poult. Sci. 2012;11:131-137.
23. Sajise JH, Madrid JA, Kohler DD. Biology of Chromolaena odorata. Philippine J. Weed Sci. 1974;1:83-87.
24. Simaraks S, Chinrasri O, Aengwanich W. Hematological, electrolyte and serum biochemical values of the Thai indigenous chickens (Gallus domesticus) in northeastern, Thailand. Songklanakarin J. Sci. Technol. 2004;26:426-430.
25. Uhegbu FO, Chibuzo CI, Onwuegbuchulam H. Lipid lowering, hypoglycemic and antioxidant activities of Chromolaena odorata L) and Ageratum conyzoides L) ethanolic leaf extracts in albino rats. J. Med. Plants Stud. 2016;42:155-159.
26. Vital PG, Windell LR. Antimicrobial activity and cytotoxicity of Chromolaena odorata L. f.) King and Robinson and Uncaria perrottetii A. Rich) Merr. Extracts. J. Med. Plants Res. 2009;37:511-518.