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The journal AGRICULTURA (A) publishes scientific works from the following fields: animal science, plant production, farm mechanisation, land management, agricultural economics, ecology, biotechnology, microbiology
ISSN 1581-5439

Livija TUŠAR, Irena Leonida KROPF, Avrelija CENCIČ

pp. 33-47


Salt is an important ingredient in the production of meat product. Any reduction of salt requires a special treatment. This study was conducted to evaluate the effect of salt reduction on the growth of microorganisms in Kranjska sausages during their shell-life and to carry out a sensory assessment. The 18 lots of sausages were prepared under salt-reduced (1.6%) and control (2.3%) salt concentrations, directly on the production line. A total of 85 sausages were analysed and the data were used for the comparisons of groups (ANOVA) and to detect the significant variables (polynomial models) influenced on the total number of microorganisms (TNMs). The significant differences were determined between the lots (representing the microbiological status of the stuffing), between the salt-reduced samples and control samples, and between the different humidity levels. The correlations and significant relationships were determined between the TNMs and the lots, the salt concentrations, and the relative humidity. The polynomial models were to general to be used for the prediction. For sensory analysis implemented on 40th day 18 sausages were assessed. The reduction of salt resulted in lower scores in the sensory evaluation. The less-salted sausages contained more microorganisms.

Key words: Kranjska sausage, reduction of salt, sensory evaluation, models, relative humidity


Vpliv zniževanja soli na vsebnost skupnega število mikroorganizmov in senzorično analizo v kranjskih klobasah v času njihovega roka uporabnosti

Sol je pomembna sestavina v proizvodnji mesnih izdelkov in vsako zniževanje vsebnosti soli zahteva posebno obravnavo. Ta študija je bila izvedena z namenom ugotavljanja vpliva zmanjševanja soli na rast mikroorganizmov v kranjskih klobasah in na senzorično oceno v času njihovega roka trajanja. 18 lotov klobas je bilo pripravljenih z zmanjšano količino (1,6%) in s kontrolno količino (2,3%) soli neposredno na proizvodni liniji. Analiziranih je bilo 85 klobas. Podatki so bili uporabljeni za primerjave skupin (ANOVA) in za odkrivanje značilnih spremenljivk (polinomski modeli), ki vplivajo na skupno število mikroorganizmov (SŠM). Signifkantne razlike so bile določene med loti Kranjskih klobas (ki predstavljajo mikrobiološko stanje nadeva), med vzorci klobas z zmanjšano količino in kontrolno količino soli in med različnimi stopnjami zračne vlage. Korelacije in signifkantna razmerja so bila določena med SŠMs in loti, koncentracijo soli v klobasi in relativno vlažnostjo. Polinomi so bili preveč splošni, da bi se lahko uporabljali za napovedovanje. Senzorična analiza je bila izvedena na 40. dan. Ocenili smo 18 klobas. Pri ocenjevanju je zmanjšanje soli prineslo klobasam nižje ocene. Manj slane klobase so vsebovale več mikroorganizmov.

Ključne besede: Kranjska klobasa, zmanjševanje soli, senzorično ocenjevanje, modeli, relativna vlažnost


1. Aaslyng MA, Vestergaard C, Koch AG. Te effect of salt reduction on sensory quality and microbial growth in hotdog sausages, bacon, ham and salami. Meat Sci. 2014;96:47-55.
2. BOX GEP, Cox DR. An analysis of transformations. J.R.Stat. Soc. Series B.Stat. Methodol. 1964;26(2):211-252.
3. Cohen J. Statistical Power Analysis for the Behavioral Sciences. Lawrence Erlbaum Associates, New Jersey, USA,1988.
4. Commission Regulation (EC) No. 2073/2005 on microbiological criteria for foodstuffs 2005. (15.11.2005).
5. Desmond E. Reducing salt: A challenge for the meat industry. MeatSci. 2006;74:188–196.
6. Desmond E. Reducing salt in meat and poultry products. In: Kilcast D, Angus F (eds.), Reducing salt in foods. Woodhead Publishing Limited, Cambridge, England, 2007:233-55.
7. Doyle ED, Glass HA. Sodium Reduction and Its Effect on Food Safety, Food Quality, and Human Health. Compr. Rev. FoodSci. FoodSaf. 2010;9:44-56.
8. Freund RJ, Littell RC. System for regression. SAS Institute Inc., Cary, NC, USA, 1991.
9. Giaouris E, Heir E, Hébraund M, Chorianopoulos N, Langsrud S, Møretrø T, Habimana O, Desvaux M, Renier S, Nychas GJ. Attachment and bioflm formation by foodborne bacteria in meat processing environments: causes, implications, role of bacterial interactions and control by alternative novel methods. Meat Sci. 2014;97:298-309.
10. Hwang CA. Development of predictive models to enhance microbiological safety of ready-to-eat meats. J. Nutr. Food Sci. 2013;3(4)70.
11. Hwang CA, Porto-Fett ACS, Juneja VK, Ingham SC, Ingham BH, Luchansky JB. Modeling the survival of Escherichia coli O157:H7, Listeria monocytogenes, and Salmonella Typhimurium during fermentation, drying, and storage of soudjouk-style fermented sausage. Int. J. Food Microbiol. 2009;129:244-252.
12. ISO 1841-2:1996. International Organisation for Standardization (ISO), Determination of chloride content – Part 2: Potentiometric method, Meat and Meat Products, Geneva, 1996.
13. ISO 4833:2003E. International Organisation for Standardization (ISO), Microbiology of food and animal feeding stuffs — Horizontal method for the enumeration of microorganisms — Colony-count technique at 30 degrees C, Food Microbiology, Geneva, 2003.
14. Kilcast D, den Ridder C. Sensory issues in reducing salt in food products. In: Kilcast D, Angus F (eds.), Reducing salt in foods. Woodhead Publishing Limited, Cambridge, England, 2007: 201-220.
15. Kloss L, Dawn Meyer J, Graeve L, Vetter W. Sodium intake and its reduction by food reformulation in the European Union — A review. NFS Journal 2015;1:9-19.
16. Kropf IL. Effects of lowering (percentage of) salt in meat products on the content of the number of microorganisms (Vpliv zniževanja soli v mesnih izdelkih na vsebnost skupnega števila mikroorganizmov) [master thesis]. Maribor. Faculty for Agriculture and Life Sciences, University of Maribor, 2012.
17. Mejlholm O, Gunvig A, Borggaard C, Blom-Hanssen J, Mellefont L, Ross T, Leroi F, Else T, Visser D, Dalgaard P. Predicting growth rates and growth boundary of Listeria monocytogenes – An international validation study with focus on processed and ready-to-eat meat and seafood. Int. J. Food Microbiol. 2010;141(3):137-150.
18. Ministry of Health, Government of the Republic of Slovenia. National Action Plan to reduce salt intake in the diet of people in Slovenia for the period 2010-2020 (Nacionalni akcijski načrt za zmanjševanje uživanja soli v prehrani prebivalcev Slovenije za obdobje 2010– 2020). 2010. (3.10.2011).
19. Montgomery DC, Peck EA. Multicollinearity in regression. In: Wadsworth HM (eds.), Handbook of statistical methods for engineers and scientists. McGraw-Hill publishing company, New York, USA, 1990:15.3-15.23.
20. Pereira AGT, Ramos EM, Teixeira JT, Cardoso GP, Ramos ALS, Fontes PG. Effects of the addition of mechanically deboned poultry meat and collagen fbers on quality characteristics of frankfurter-type sausages. Meat Sci. 2011;89:519-525.
21. Puolanne E, Halonen M. Teoretical aspects of waterholding in meat. Meat Sci. 2010;86(1):151-161.
22. Institute of public health of the Republic of Slovenia in the cooperation with the Slovene Consumers’ association. Guidelines for microbiological safety, which are intended for the fnal consumer (Smernice za mikrobiološko varnost živil, ki so namenjena končnemu potrošniku). Authors:Rupel T, Pavlica T, Planina P, Vidrih J, Retelj M, Car T, Gošnjak M, Lušicky M, Bošnjak D, Pretnar G, Sabotin D., 2009.
23. Sharedeh D, Gatellier P, Astruc T, Daudin JD. Effects of pH and NaCl levels in a beef marinade on physicochemical states of lipids and proteins and on tissue microstructure. Meat Sci. 2015;110:24-31.
24. Slovenian Environment Agency. Whether information for Maribor in year 2011. .
25. Soladoye OP, Juárez ML, Aalhus JL, Shand P, Estévez M. Protein Oxidation in Processed Meat: Mechanisms and Potential Implications on Human Health. Compr. Rev. Food Sci. Food Saf. 2015;14:106-122.
26. SAS 9.4 (TS1M1) for Windows. SAS Institute Inc., Cary, NC, USA, 2002-2012.
27. SAS/STAT® 9.2 User’s Guide. SAS Institute Inc., Cary, NC, USA, 2008.
28. World Health Organization(WHO). Diet, nutrition and the prevention of chronic diseases. Report of a Joint WHO/FAO Expert Consultation, Who Technical Report Series, Geneva, 2002. TRS_916.PDF (28.1.-1.2.2002).
29. Winsberg S, Ramsay JO. Monotonic transformations to additivity using splines. Biometrika1980;67(3):669-674.

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