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The journal AGRICULTURA (A) publishes scientific works from the following fields: animal science, plant production, farm mechanisation, land management, agricultural economics, ecology, biotechnology, microbiology
ISSN 1581-5439

Etleva VEIZAJ, Myqerem TAFAJ, Klaus MÄNNER
pp. 37-41

A combined probiotic preparation of Lactobacillus plantarum ATCC 14917 1x1011 CFU/kg, Lactobacillus fermentum DSM 20016 1x1011 CFU/kg and Enterococcus faecium ATCC 19434 1x1011 CFU/kg was supplemented to a basal ration with 100, 150 and 200 mg/kg feed and the eff ects on growth performance, apparent nutrient digestibility, digesta pH and the total number of microbes (Anaerobe bacteria, Lactobacilli spp, Enterococci spp, Escherichia coli) of defined intestinal segments on thirty two weaned piglets (28 days) were studied for six weeks experimental period. The supplementation of combined probiotic improved slightly daily weight gain (DWG) and feed conversion ratio (FCR), kg feed/kg weight gain. Fibre digestibility was slightly increased and fat digestibility was slightly decreased. Overall a positive eff ect of the probiotic on growth performance was observed. Th e results indicate that the probiotic preparation may be less suppressive to the Escherichia coli. Th e eff ects of the probiotic preparation on the microbial composition of the chyme showed no dose–depended eff ects. Failure of a dose-response and not clear eff ects on pH, digestibility, microbial composition in faeces and chyme were possibly caused by combination of diff erent strains. However we recommend the level of 150 mg/kg feed combined probiotic as the optimal dose.

Key words: combined probiotic, microbial composition, digesta, mucosa, faeces, weaned piglet


Vpliv kombiniranega probiotika na intenzivnost rasti, prebavljivost in na mikrobno sestavo črevesja ter blata odstavljenih pujskov

Kombinirani probiotični pripravek Lactobacillus plantarum ATCC 14917 1x1011 CFU/kg, Lactobacillus fermentum DSM 20016 1x1011 CFU/kg in Enterococcus faecium ATCC 19434 1x1011 CFU/kg je bilo dodanih v bazalni obrok v količini 100, 150 in 200 mg/kg krme. Proučevane lastnosti so bile intenzivnost rasti, navidezna prebavljivost hranil, pH vrednost, skupno število mikroorganizmov (bakterij, Lactobacili spp, enterokoki spp, Escherichia coli) v določenih delih tankega črevesja pri dvaintridesetih odstavljenih pujskih (28 dni). Čas testiranja je bil šest tednov. Dopolnitev obroka s kombiniranim probiotikom nekoliko izboljšala velikost dnevnega prirasta (DWG) in učinkovitost izkoriščanja krme (FCR). Prebavljivost vlaknin se je nekoliko povečala in prebavljivost maščob se je nekoliko znižala. Ugotovljen je bil skupni pozitivni učinek probiotika na intenzivnost rasti prašičev. Rezultati so pokazali, da probiotik deluje manj zaviralno na Escherichia coli. Probiotik ni pokazal vpliva na mikrobiološko sestavo želodčne vsebine in količina odmerka prav tako ni imela nobenega vpliva. Odpoved delovanja probiotika in odmerka na pH vrednost, prebavljivost, mikrobno sestavo v blatu in želodčno vsebino ni pojasnjena, vendar domnevamo, da je razlog v različnih sevih mikroorganizmov. Vseeno je priporočen odmerek dodatka probiotika v krmo prašičev 150 mg/kg krme.

Ključne besede: kombinirani probiotik; mikrobna sestava; prebava, sluznica, blato, odstavljeni pujski


1. PA, Brooker BE, Fuller R, Newport MJ. The attachment of bacteria to the gastric epithelium of the pigs and its importance in the microecology of the intestine. J. Appl. Bacteriol. 1980; 48: 147-154.

2. Benno J, Teakwang O. Research and developments of probiotics for animal production. In: Symposium on the Asian Network on Microbial Research. Microbial Sources and their utilization on Asia, Chiba, Japan. 2001;55 (2): 105-111,

3. Breves G, Walter C, Burmeister M, Shröder B. In vitro studies on the eff ects of Saccharomyces boulardii and Bacillus cereus var. toyoi on nutrient transport in pig jejunum. J. Anim. Physiol and Anim Nutrition. 2000;84: 9-20.

4. Brooks PH, Beal JD, Dmeckova V, Niven S. Probiotics for pigs and beyond. In: Van Vooren and B. Rochet. Role of probiotics in animal nutrition and their link to the demands of Europian consumers, ID-Lelystad, 2003;49-59.

5. De Cupere F, Deprez P, Demeulenaere D, Muylle E. Evaluation of the eff ect of 3 probiotics on experimental Escherichia coli enterotoxaemia in weaned piglets. J. Vet. Med. Bulletin. 1992;39: 277-284.

6. Gedek K, Kirchgessner M, Wiehler S, Bott A, Eidelsburger U, Roth FX. Zum Einfluß von ameisensäure auf die Keimzahlen der Segmenten des Gastrointestinaltraktes. J. Anim. Physiol. and Anim. Nut. 1992;68: 206-217.

7. Gedek K, Kirchgessner M, Wiehler S, Bott A, Eidelsburger U, Roth FX. Zur Nutritiven Wirksamkeit von Bacillus cereus als probiotikum in der ferkelaufzucht. Archiv Anim. Nut. 1993;44: 215-226.

8. Goebel S, Vahjen W, Jadamus A, Simon O. PCR assay for detection of porcine pathogenic Escherichia coli virulence factors in the gastrointestinal tract of piglets fed a spore forming probiotic. Proc. 9th Society for Nutrition and Physiology. 2000; 64.

9. Hale OM, Newton KI. Effetcs of a nonviable Lactobacillus species fermentation product on performance of pigs. J. Anim. Sci. 1979;48(4):770-775

10. Jadamus A, Vahjen W, Simon O. Influence of the probiotic bacterial strain, Bacillus cereus var. toyoi on the development of selected microbial groups adhering to intestinal mucosal tissues of piglets. J. Anim. Feed Sci. 2000;9: 347-362.

11. Jadamus A. Untersuchungen zur Wirksamkeit und Wirkungsweise des sporenbildenden Bacillus cereus var. toyoi im Verdauungstrakt von Broilern und Ferkel. Degree Dissertation, Free University, Berlin 2001.

12. Jadamus A, Vahjen W, Simon O. Growth behaviour of a spore forming probiotic strain in the gastrointestinal tract of broiler chicken and piglets. Archiv of Anim. Nut. 2001;54: 1-17.

13. Lessard M, Brisson GJ. Eff ect of Lactobacillus Fermentation Product on growth immume response and fecal enzyme activity in weaned pigs. Can. J. Anim. Sci. 1987;67: 509-516.

14. Männer K, Spieler A. Probiotics in piglets, an alternative to traditional growth promoters. Microecology and Th erapy. 1997;26: 243-256.

15. Männer K, Jadamus A, Vahjen W, Frackenpohl U, Simon O. Effekte probiotischer Zusätze auf Leistungsparameter und intestinale Mikroflora. Proc. 7. Tagung, Schweine und Gefl ügelernährung, 2002;78-80.

16. Morelli L. Variations of Coliformes and Lactobacilli spp content in liquid or soft faeces belonging at swines treaties and not treaties. Published by AKRON-firm. 1995.

17. Pollmann DS, Danielson DM, Peo ER. Effects of microbial feed additives on performace of starter and growing-finishing pigs. J. Anim. Sci. 1980;51(3): 577-581.

18. Simon O, Vahjen W, Scharek L. Microorganisms as Feed Additive-Probiotics. Proc. 9 th International Symposium on Digestive Physiology in Pigs, Banff , Canada.2003;1: 295-318.

19. Solano-Aguilar GI, Vengroski KG, Beshah E, Lunney JK. Isolation and purifi cation of lymphocyte subset from gut-associated lymphoid tissue in neonatal swine. Immunological Methods 2000;241: 185-199.

20. Tortuer OF. Influence of implantation of Lactobacillus acidophilus in chicks on the growth, feed conversion, malabsorption of fats syndrome and intestinal flora. Poult. Sci. 1973;2: 197-203.

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