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The journal AGRICULTURA (A) publishes scientific works from the following fields: animal science, plant production, farm mechanisation, land management, agricultural economics, ecology, biotechnology, microbiology
ISSN 1581-5439
Manoharan Melvin JOE and Palanivel Karpagavinayaga SIVAKUMAAR
pp. 47-51

The response of Azorhizobium caulinodans to reactive oxygen species such as hydrogen peroxide (H2O2) plays an important role in nodulation and nitrogen fixation. It has been found that pretreatment of A.caulinodans with a selective oxidant viz., H2O2 (200μM) has enhanced the osmotic, thermal, and desiccation tolerence. The survivability in different carrier materials was found to be enhanced by H2O2 pretreatment as compared to untreated controls. A pot culture study was conducted to compare the effi ciency of H2O2 pretreated cells for nodulation, plant dry wt and ARA activity in Sesbania rostrata, followed by the Sesbania incorporation as green manure for rice grown under pot culture condition. The crop was studied for certain plant growth and yield components, such as plant ht, plant dry wt, no of panicles, total “N” uptake. The results of our present study have showed that the plant inoculated with H2O2 pre-treated cells of A. caulinodans positively augmented an increase in the growth and yield in both Sesbania rostrata as well for rice grown under flooded conditions in the pot culture experiment.

Key words: Azorhizobium caulinodans, hydrogen peroxide, carrier material, rurvival, rice


Slovenian:

Rast in fiksacija N2 v Sesbania rostrata s H2O2 predhodno tretiranim Azorhizobium caulinodans in njegov učinek kot zeleni gnoj na nižinski riž

Odziv Azorhizobium caulinodans na proste radikale, kot so vodikov peroksid (H2O2) ima pomembno vlogo pri nodulaciji in fiksaciji dušika. Ugotovljeno je bilo, da je predhodna obdelava s A. caulinodans s selektivnim oksidantom H2O2 (200 µm) izboljšala osmotsko, toplotno in izsušitveno toleranco. Preživetje je bilo ugotovljeno v različnih nosilnih materialih in okrepljeno s H2O2 pred obdelavo. Študija v posodah je bila izvedena za primerjavo učinkovitosti predhodnega tretmaja s H2O2 obdelanih celic za nodulacijo, suhe snovi rastlin in ARA dejavnosti v Sesbania rostrata. Sesbania rostrata je bila vključena v zeleno gnojilo za gojitev riža v posodi. Proučevana je bila rast rastlin in pridelek, kot so velikost rastline, suha snov rastlin, late in skupni "N" odvzem. Rezultati študije so pokazali, da rastlina z inokulacijo s H2O2 predhodno obravnavanih celic A. caulinodans pozitivno poveča rast in pridelek v obeh tako v Sesbania rostrata kot pri rižu gojenem v pogojih poplavnih razmer v poskusu v loncih.

Ključne besede: Azorhizobium caulinodans; vodikov peroksid; nosilni material; preživetje; riž


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